UVAS Short Training by IBBT on “Basic Molecular Biology Techniques
Molecular biology deals with the molecular basis of biological activity. Molecular biology chiefly concerns itself with understanding the interactions between the various systems of a cell, including the interactions between the different types of DNA, RNA and protein biosynthesis as well as learning how these interactions are regulated. The basic techniques of molecular Biology are DNA extraction from variety of source samples, Polymerase Chain Reaction (PCR), and Real Time PCR. DNA extraction is the removal of deoxyribonucleic acid (DNA) from viruses or other cells to perform a number of processes including the sequencing of genomes, detection of bacteria and viruses in the environment, detection of diseases, and many other forensic and diagnostic processes. The polymerase chain reaction (PCR) is a biochemical technology in molecular biology used to amplify a single or a few copies of a piece of DNA across several orders of magnitude, generating thousands to millions of copies of a particular DNA sequence. A quantitative polymerase chain reaction (qPCR), also called real-time polymerase chain reaction, is a laboratory technique of molecular biology based on the polymerase chain reaction (PCR), which is used to amplify and simultaneously quantify a targeted DNA molecule. These techniques have widespread applications in many areas of genetic analysis such as Medical applications, Infectious disease applications, Forensic applications and research application.
Any PCR users who have an interest in starting to use real-time PCR or current new users who want further training on Gene Expression Applications: Absolute and Relative Quantitation, Theory, Analysis and Optimization of PCRs.
|09:00 AM-09:15 AM:||Introduction of the course by Prof. Dr. Tahir Yaqub|
|09:15 AM-10:15 AM:||Lecture on DNA Extraction by Dr. Asif Nadeem /Dr. Wasim Shehzad|
|10:15 AM-1:00PM:||Practical Training of DNA Extraction: From Blood Samples by Ms. Maryam Javed.
From Tissue Samples by Dr. Sehrish Firyal / Ms. Lala Rukh.
|9:00 AM-10:00 AM:||Lecture on Polymerase Chain Reaction (PCR) by Dr. Ali Raza Awan/Dr. M. Imran|
|10:00 AM-12:00 PM:||Practical Training of Polymerase Chain Reaction (PCR) by Dr. M. Yasir Zahoor/Dr. M. Imran|
|09:00 AM-10:00 AM:||Lecture on Real Time PCR (RT-PCR) by Dr. M. Zubair Shabir/Dr. M. Wasim|
|10:00 AM-1:00 PM:||Practical Training of Real Time PCR (RT-PCR) Session I by Dr. M. Zubair Shabir/Dr. M. Nauman Zahid|
|2:00 PM-3:30 PM:||Practical Training of Real Time PCR (RT-PCR) Session II by Dr. M. Zubair Shabir /Mr. Rashid Saif.|
|3:30 PM 4:00 PM:||Concluding Session and Certificate Distribution Ceremony|
At the end of the course the attendee will be familiar with the theory and practice of PCR and real time PCR and one will learn the most cost-effective way to set-up, run and analyze the basic molecular biology experiments.
Laste Date of Registration : 30th June 2014
Institute of Biochemistry and Biotechnology
University of Veterinary and Animal
Prof. Dr. Tahir Yaqub
Institute of Biochemistry & Biotechnology
University of Veterinary and Animal Sciences
Dr. Ali Raza Awan
Dr. M. Yasir Zahoor