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UVAS Short Training by IBBT on “Basic Molecular Biology Techniques

UVAS Short Training By IBBT On “Basic Molecular Biology Techniques

UVAS Short Training by IBBT on “Basic Molecular Biology Techniques

Introduction

Molecular biology deals with the molecular basis of biological activity. Molecular biology chiefly concerns itself with understanding the interactions between the various systems of a cell, including the interactions between the different types of DNA, RNA and protein biosynthesis as well as learning how these interactions are regulated. The basic techniques of molecular Biology are DNA extraction from variety of source samples, Polymerase Chain Reaction (PCR), and Real Time PCR. DNA extraction is the removal of deoxyribonucleic acid (DNA) from viruses or other cells to perform a number of processes including the sequencing of genomes, detection of bacteria and viruses in the environment, detection of diseases, and many other forensic and diagnostic processes. The polymerase chain reaction (PCR) is a biochemical technology in molecular biology used to amplify a single or a few copies of a piece of DNA across several orders of magnitude, generating thousands to millions of copies of a particular DNA sequence. A quantitative polymerase chain reaction (qPCR), also called real-time polymerase chain reaction, is a laboratory technique of molecular biology based on the polymerase chain reaction (PCR), which is used to amplify and simultaneously quantify a targeted DNA molecule. These techniques have widespread applications in many areas of genetic analysis such as Medical applications, Infectious disease applications, Forensic applications and research application.

Who should attend?

Any PCR users who have an interest in starting to use real-time PCR or current new users who want further training on Gene Expression Applications: Absolute and Relative Quantitation, Theory, Analysis and Optimization of PCRs.

Objectives

  • To better understand the basic techniques of Molecular Biology
  • To update the participants with latest applications of DNA and PCR
  • To demonstrate real-time PCR for understanding of its applications in academia and industry
  • To promote the usage of basic Molecular Biology techniques in life sciences research

Program

Tuesday 08th July, 2014

09:00 AM-09:15 AM: Introduction of the course by Prof. Dr. Tahir Yaqub
09:15 AM-10:15 AM: Lecture on DNA Extraction by Dr. Asif Nadeem /Dr. Wasim Shehzad
10:15 AM-1:00PM: Practical Training of DNA Extraction: From Blood Samples by Ms. Maryam Javed.
From Tissue Samples by Dr. Sehrish Firyal / Ms. Lala Rukh.

Wednesday 09th July, 2014

9:00 AM-10:00 AM: Lecture on Polymerase Chain Reaction (PCR) by Dr. Ali Raza Awan/Dr. M. Imran
10:00 AM-12:00 PM: Practical Training of Polymerase Chain Reaction (PCR) by Dr. M. Yasir Zahoor/Dr. M. Imran

Thursday 10th July, 2014

09:00 AM-10:00 AM: Lecture on Real Time PCR (RT-PCR) by Dr. M. Zubair Shabir/Dr. M. Wasim
10:00 AM-1:00 PM: Practical Training of Real Time PCR (RT-PCR) Session I by Dr. M. Zubair Shabir/Dr. M. Nauman Zahid
2:00 PM-3:30 PM: Practical Training of Real Time PCR (RT-PCR) Session II by Dr. M. Zubair Shabir /Mr. Rashid Saif.
3:30 PM 4:00 PM: Concluding Session and Certificate Distribution Ceremony

Goals

At the end of the course the attendee will be familiar with the theory and practice of PCR and real time PCR and one will learn the most cost-effective way to set-up, run and analyze the basic molecular biology experiments.

Contents

  • DNA Extraction
  • Polymerase Chain Reaction
  • Real Time PCR
  • See Detailed Contents

Fees

  • Rs. 4,000 for faculty/entrepreneurs
  • Rs 2,000 for students
  • Lunch and tea will also be served

 

Brochure

Course Programme

Registration Form

Laste Date of Registration : 30th June 2014

Venue

Institute of Biochemistry and Biotechnology
(IBBT)
University of Veterinary and Animal
Sciences, Lahore

Contact

Prof. Dr. Tahir Yaqub
Director
Institute of Biochemistry & Biotechnology
University of Veterinary and Animal Sciences
Lahore-Pakistan
0300-6950418
042-99210256

Dr. Ali Raza Awan
Assistant Professor
(0321-8442090)
arawan77@uvas.edu.pk

Dr. M. Yasir Zahoor
Assistant Professor
yasir.zahoor@uvas.edu.pk

UVAS Short Training By IBBT On “Basic Molecular Biology Techniques

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